Philippine E-Journals

Antimitotic and Antiangiogenic Assay of Fucoidan from Sargassum oligocystum

Richie G. Bayuran

Discipline: Biology

 

Abstract:

Algae are the large group of autotrophic organisms that do not have organized cell structures but have unique biological and pharmacological activities. Mitosis and angiogenesis are standard biological processes that needed in the maintenance of life. The inappropriate activity of these processes can lead to severe complications like cancer. Due to the abundance of algal species in Panay, Philippines. This study determined the antimitotic and antiangiogenic activities of fucoidan from Sargassum oligocystum. Sargassum oligocystum was extracted with hot distilled water (80o C for 4 hours) and subjected to lyophilization which yielded an average of 7.67% fucoidan from Aklan, Antique and Capiz brown algae. Fucoidan showed identical spectra when compared to the reference standard using FTIR Spectrometer at a wavelength of 650 to 4000cm-1. Antimitotic assay revealed that 100mg/ml of fucoidan solution has a significant result in mitotic index and the effectiveness was comparable with Methotrexate. Likewise, 100mg and 10 mg of fucoidan in gelatin disc exhibited a reduction in the number of branches and entire branches length of the blood vessel, and the effectiveness was comparable to Retinoic acid. With distinction among the treatments, 100mg of Fucoidan is a promising therapeutic agent because it showed antimitotic and antiangiogenic activities.