Philippine E-Journals

Wound Healing Activity of Aqueous Extracts of Common Legumes

Jinky Marie T. Chua

 

Abstract:

The study aimed to investigate the wound healing activity of the commonly cultivated legumes in the Philippines, Dolichos lablab, Phaseolus lunatus, and Glycine soja. In the present study, the aqueous extracts of the seeds were administered orally to the Sprague Dawley rats. Evaluation of wound healing activity was done using the incision and excision wound models. In each model, group I served as the control group, group II as the test group treated with Dolichos lablab extract, group III as the group treated with Phaseolus lunatus extract and group IV with Glycine soja extract. Incision wound model rats were treated for ten days, and the wound healing activity was assessed by the tensile strength and histopathological studies of the granulation tissue. Excision wound model rats were treated until the wounds were closed entirely. In this model, wound healing activity was assessed by the rate of wound closure and free radical scavenging activity was analyzed using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method. In both models, hydroxyproline content in the scab was determined. The results showed that the extract of Dolichos lablab possessed better-wound healing and antioxidant activity than Phaseolus lunatus and Glycine soja extracts.