Biogenic Synthesis of Silver Nanoparticles using Philippine Lime (Citrofortunella microcarpa) Peel Extract and its Antibacterial Activity in Comparison to Ciprofloxacin against Escherichia coli

Irene  V. Columbano,; Sarang  M. Chaudary,; Chiari Emilie  I. Durana,; Aira Louise  E. Empaynado,; Shairyll Queenie  R. Fadriquelan,; Anne Claire  F. Feudo,; Jennilyne  G. Fule,; Manali  C. Kamerkar,; Pankti  J. Kanani,; Pardeep  Kaur,; Beverly Grace  A. Lamug,; Janina  N. Lastimosa,

Biogenic Synthesis of Silver Nanoparticles using Philippine Lime (Citrofortunella microcarpa) Peel Extract and its Antibacterial Activity in Comparison to Ciprofloxacin against Escherichia coli

Abstract:

Objective: The main objective of this study is to determine the effectiveness of Philippine lime as a reducing agent in synthesizing silver nanoparticles (AgNps) to produce antimicrobial properties against Escherichia coli. Specifically, it aims to determine the effectiveness of synthesized AgNPs against Escherichia coli using minimum inhibitory concentration (MIC) disk diffusion and serial dilution then comparing its effectiveness against the standard drug ciprofloxacin. Methods: This study is an experimental research design and was conducted at Manila Central University Laboratory. The AgNP was synthesized from 1mM of silver nitrate by using 5mL of Philippine lime peel extract as a reducing agent. The initial characterization of the formed AgNPs was done by Ultraviolet-visible (UV-Vis) spectroscopy, the particle size was determined by Scanning Electron Microscope (SEM), and the elemental composition was examined through Energy Dispersive X-Ray (EDX) analysis. Statistical treatment data was applied using One-Way Analysis of Variance and Welch F-test followed by Tukey’s pairwise post-hoc tests with significance limit set at 5% probability. Results: The antimicrobial property of AgNP solution with varying volumes of 50uL, 75uL, and 100uL were shown to have no activity against Escherichia coli using standard values. Conclusion: Citrofortunella microcarpa peel extract has been proven effective in reducing silver ions into AgNP. There was zero inhibition compared to ciprofloxacin which obtained the standard zone of inhibition.