Home⇛Annals of Tropical Research⇛vol. 40 no. 1 (2018)

Azolla pinnata R.Br.: a fern species that demonstrates satisfactory invitro antioxidation under herbicidal toxicity

Arnab Kumar De | Indraneel Saha | Bipul Sarkar | Narottam Dey | MK Adak

 

Abstract:

The paper reports the induced antioxidation property of Azolla pinnata R.Br. under elevated conditions of the herbicide 2,4-D. With respect to phenolic accumulation, the plant registered varied in-vitro antioxidation potentials. Total antioxidation through phosphomolybdenum complex, DPPH (2,2-Diphenyl, 1- picryllydrazyl) and ABTS (2,2'-azino-bis 3-ethylbenzo-thiazoline-6-sulfonic acid) radical scavenging activities were the most significant attributes of this plant species. A gradual fall in phosphomolybdenum complex suggested more involvement in the chelation of 2,4-D with metal ligands. The plant was able to quench the reactive oxygen species (ROS) up to a certain level of 2,4-D but thereafter it failed. Another module with ABTS induced free radical antioxidation, the plant insignificantly responded to any changes of 2,4-D concentrations as compared to the control with reference to BHT (Butylated hydroxytoluene). The most stable phenolic glucoside as flavonoid had a significant and dose-dependent over expression under 2,4-D toxicity. In modules of enzymatic antioxidants, Azolla was quite sensitive to peroxidation of H20 2 by different isozymic proteins. A significant participation of polyphenol oxidase and catalase were more pronounced whereas peroxidases were least significant in in vitro assay. Taking altogether both enzymatic and non-enzymatic antioxidation indices, Azolla is an efficient quencher species for herbicide contaminated soils.



References:

  1. Aebi H. 1984. Catalase in vitro, Methods in Enzymology, 105:121-126.
  2. Agbor GA, Kuate D & Oben JE. 2007. Medicinal plants can be good source of antioxidants: case study in Cameroon. Pakistan J Biological Science, 10:537-544
  3. Ahuja I, Kissen R & Bones AM. 2012. Phytoalexins in defense against pathogens. Trends in Plant Science, 17:73-90.
  4. Amarowicz R., R. Pegg RB, Rahimi-Moghaddam P, Barl B & Weil JA. 2004. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chemistry, 84:551-562.
  5. Ammar WB, Nouairi I, Zarrouk M, Ghorbel MH & Jemal F. 2008. Antioxidative response to cadmium in roots and leaves of tomato plants. Biologia Plantarum, 52:727-731,
  6. Bhaskaran SK and Kannappan P. 2017. Protective effect of Azolla microphylla on biochemical, histopathological and molecular changes induced by isoproterenol in rats, Biomedicine & Pharmacotherapy, 89:473-481.
  7. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181:1199-1200.
  8. Dai LP, Dong XJ & Ma HH. 2012. Molecular mechanism for cadmium-induced anthocyanin accumulation in Azolla imbricata. Chemosphere, 87:319-325.
  9. Das K and Roychoudhury A. 2014. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science, 2:53.
  10. De AK, Dey N & Adak MK. 2016. Some physiological insights of 2,4-D sensitivity in an aquatic fern: Azolla pinnata R.Br. J Biotechnology Biomaterials, 6:235.
  11. De AK, Sarkar B & Adak MK. 2017. Physiological explanation of herbicide tolerance in Azolla pinnata R. Br. Annals of Agrarian Science (2017), http://dx.doi.org/10.1016/j.aasci.2017.05.021.
  12. De AK, Dey N & Adak MK. 2016. Bio indices for 2, 4-D sensitivity between two plant species: Azolla pinnata R. Br. and Vernonia cinerea L. with their cellular responses. Physiology & Molecular Biology of Plants, 22:371-380.
  13. De AK, Dey N & Adak MK. 2016. Biotechnological Implication with Azolla pinnata R. Br. for Metal Quenching Ability with Physiological Biomarkers. Cryptogam Biodiversity & Assessment 1, no. 1 (2016).
  14. Dhir B. 2013. Phytoremediation: Role of aquatic plants in environmental clean-up. Springer Science & Business Media.
  15. Farkas MH. 2008. Plant processes that affect the phytotoxicity of pharmaceutical contaminants in the environment. State University of New York at Buffalo.
  16. Hartmann T. 2007. From waste products to ecochemicals: fifty years research of plant secondary metabolism. Phytochemistry, 68:2831-2846.
  17. Jiang Y, Zhang Z, Joyce DC & Ketsa S. 2002. Postharvest biology and handling of longan fruit (Dimocarpus longan Lour.). Postharvest Biology & Technology, 26:241-252.
  18. Kang SH. 2013. Exploring the Mechanisms of Desiccation Tolerance in Resurrection Plants: Toward Developing Desiccation Tolerance Crops. University of Nevada, Reno.
  19. Kaur P, Kumar M, Pal Singh A & Kaur S. 2017. Ethyl acetate fraction of Pteris vittata L alleviates 2-acetylaminofluorene induced hepatic alterations in male Wistar rats. Biomedicine & Pharmacotherapy, 88:1080-1089.
  20. Kikuchi T and Tanaka S. 2012. Biological removal and recovery of toxic heavy metals in water environment. Critical Reviews in Environmental Science & Technology, 42:1007- 1057.
  21. Mandal C, Ghosh N, Adak MK & Dey N. 2013. Interaction of polyamine on oxidative stress induced by exogenously applied hydrogen peroxide in Salvinia natans Linn. Theoretical & Experimental Plant Physiology, 25:223-230.
  22. Martínez JP and Araya H. 2010. Ascorbate-glutathione cycle: enzymatic and non- enzymatic integrated mechanisms and its biomolecular regulation. Ascorbate-glutathione pathway and stress tolerance in plants (pp303-322). Springer Netherlands.
  23. Molina L and Kahmann R. 2007. An Ustilago maydis gene involved in H₂O, detoxification is required for virulence. The Plant Cell, 19:2293-2309,
  24. Murashige T and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarium, 15:473-497.
  25. Parmar S and Singh V. 2015. Phytoremediation approaches for heavy metal pollution: a review. J Plant Science Research, 2:135.
  26. Prieto P, Pineda M & Aguilar M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry, 269:337-341.
  27. Rai PK. 2009. Heavy metal phytoremediation from aquatic ecosystems with special reference to macrophytes. Critical Reviews in Environmental Science & Technology, 39:697-753.
  28. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M & Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26:1231-1237.
  29. Shahidi F and Zhong Y. 2010. Lipid oxidation and improving the oxidative stability. Chemical Society Review,s 39:4067-4079.
  30. Sheo MP, Singh A & P. Singh P. 2015. Physiological, biochemical and growth responses of Azolla pinnata to chlorpyrifos and cypermethrin pesticides exposure: a comparative study, Chemistry & Ecology, 31:285-298.
  31. Subramanian R, Raj V, Manigandan K & Elangovan N. 2015. Antioxidant activity of hopeaphenol isolated from Shorea roxburghii stem bark extract." J Taibah University for Science, 9: 237-244.
  32. Velioglu YS, Mazza G, Gao L & Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agricultural & Food Chemistry, 46:4113-4117.
  33. Zhishen J, Tang M & Wu J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64:555-559.

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