Comparative DNA Analysis of Coconut (Cocos nucifera L.) palms with polyembryonic and monoembryonic origins

Daisy Jane  A. Toting,; Dilberto  O. Ferraren,; Tessie  C. Nuñez,

Comparative DNA Analysis of Coconut (Cocos nucifera L.) palms with polyembryonic and monoembryonic origins

Daisy Jane A. Toting | Tessie C. Nuñez | Dilberto O. Ferraren

Abstract:

Makapuno is a rare, high-value coconut in the Philippines known for its extraordinary thick gelatinous meat with various uses in the food industry. Homozygous makapuno embryos do not germinate in vivo so plantlets are produced in vitro ne plantlet embryo. where o grows from an Rare cases of polyembryony were observed in makapuno hybrids developed by the Visayas State University. Knowledge of the genetic control of polyembryony may be used to increase the production of planting materials of these rare coconut types. DNA analysis of two sets of twins (polyembryonic), three monoembryonic hybrid palms, and their monoembryonic parental cultivars Coconiño and tall makapuno was done using seven DNA primers to determine differences which may be associated with polyembryony in the hybrids. Polyacrylamide Gel Electrophoresis of PCR products showed DNA fragments amplified by primers CAC2 and CAC56 which are unique to the twins suggesting that polyembryony might have a genetic origin.

References:

Joshi SP, Ranjekar PK & Gupta VS. 1999. Molecular markers in plant genome analysis (pp1-19). http/www.ias.ac.in/currsci/jul25/articles15.htm
Lantaca LD. 2016. PCR-based Comparison of Twin and Normal Makapuno (BS thesis). Visayas State University, Baybay City, Leyte, Philippines
Nuñez TC and Ocoy ET. 2008. Polyembryony in Philippine Makapuno (Cocos nucifera L.) Hybrids. Coconut Research & Development Journal 24(2):40-46
Perera L, Russel JR, Provan J & Powell W. 1999. Identification and characterisation of microsatellites in coconut (Cocos nucifera L.) and the analysis of coconut populations in Sri Lanka. Molecular Ecology 8(2):344-346
Powell W, Morgante M & Andre R. 1996. The comparison of RFLP, RAPD, AFLP, and SSR (microsallite) markers for germplam analysis. Molecular Breeding 2(3):225-238
Quimio CA, Galambao MB, Armenia AR & Bitanctor E. 2001. Development of DNA markers for identification and utilization of outstanding tall populations and hybrids to accelerate coconut replanting. Unpublished Research Project. Visayas State University
Rivera RL. 2012. PCA spurs coco production to mass-produce natural sweetener. http://www.gmanetwork.com/news/story/243716/money/pca-spurs-coco-production-to- mass-profuce-natural-sweetener#sthash.ksD3HrYa.dpufr
Rodriguez MA, Monter AV, Castaneda GC & Velazquez AG. 2004. Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD. Pesquisa Agropecuária Brasileira 39(6):551-559
Tautz D and Renz M. 1984. Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Research 12(10):4127-4138
Zuñiga LC. 1953. The probable inheritance of the makapuno character of coconut.