Ultrastructure and fourier transform infrared spectrum of exopolysaccharide produced by Lactobacillus rhamnosus NBRC 3425
Ivy C. Emnace
Abstract:
In the search for exopolysaccharide (EPS)-producing LAB strains as potential
additive for industrial applications, EPS produced by
NBRC 3425 was subjected to partial characterization to provide further information
on its structure and composition. The crude EPS was subjected to scanning
electron microscopy (SEM), atomic force microscopy (AFM), transmission electron
microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results of
TEM analysis confirmed that the test organism is an EPS producer due to the
presence of an unstained, clear cell wall or halo that surrounds the bacterial cell
typical of a capsular EPS. SEM analysis showed that the crude EPS has pores and
spaces between particles. Atomic force microscopy (AFM) at a concentration of
10μg per mL revealed spike-shaped lumps with an average size of 17.81±2.89nm.
The FTIR spectrum suggested the presence of hydroxyl (OH) groups of
carbohydrate and carbonyl group (C=O). Results showed that based on its
structural characteristics, such EPS has the potential for use as stabilizer in food
products.
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