Home⇛Annals of Tropical Research⇛vol. 42 no. 1 (2020)

Ultrastructure and fourier transform infrared spectrum of exopolysaccharide produced by Lactobacillus rhamnosus NBRC 3425

Ivy C. Emnace

 

Abstract:

In the search for exopolysaccharide (EPS)-producing LAB strains as potential additive for industrial applications, EPS produced by NBRC 3425 was subjected to partial characterization to provide further information on its structure and composition. The crude EPS was subjected to scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results of TEM analysis confirmed that the test organism is an EPS producer due to the presence of an unstained, clear cell wall or halo that surrounds the bacterial cell typical of a capsular EPS. SEM analysis showed that the crude EPS has pores and spaces between particles. Atomic force microscopy (AFM) at a concentration of 10μg per mL revealed spike-shaped lumps with an average size of 17.81±2.89nm. The FTIR spectrum suggested the presence of hydroxyl (OH) groups of carbohydrate and carbonyl group (C=O). Results showed that based on its structural characteristics, such EPS has the potential for use as stabilizer in food products.



References:

  1. Abbad-Andaloussi S, Talbaoui H, Marczak R & Bonaly R. 1995. Isolation and characterization of exocellular polysaccharides produced by Bifidobacterium longum. Applied Microbiology and Biotechnology 43(6):995-1000
  2. Coates J. 2000. Interpretation of Infrared Spectra, A Practical Approach. In Meyers RA (ed) Encyclopedia of Analytical Chemistry (pp10815-10837). John Wiley & Sons Ltd, Chichester
  3. Florou-Paneri P, Christaki E & Bonos E. 2013. Chapter 25 in Lactic acid bacteria as source of functional ingredients. In Kongo JM (ed) Lactic Acid Bacteria - R& D for Food, Health and Livestock Purposes. InTechOpen
  4. Ganzle MG and Schwab C. 2009. Ecology of exopolysaccharide formation by lactic acid bacteria: Sucrose utilisation, stress tolerance, and biofilm formation. In Ullrich M (ed) Bacterial Polysaccharides: Current Innovations and Future Trends. Caister Academic Press, Bremen, Germany
  5. German B, Schiffrin EJ, Reniero R, Mollet B, Pfeifer A & Neeser JR. 1999. The development of functional foods: Lessons from the gut. Trends in biotechnology 17:492- 499
  6. Gibson R and Roberfroid MB. 1995. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. The Journal of Nutrition 125(6):1401-1412
  7. Gutnick Dl and Bach H. 2000. Engineering bacterial biopolymers for the biosorption of heavy metals; new products and novel formulations. Applied Microbiology and Biotechnology 54:451-460
  8. Ha YW, Stack R, Hespell RB, Gordon SH & Bothast RJ. 1991. Some chemical and physical properties of extracellular polysaccharides produced by Butyrivibrio fibrisolvens strains. Applied and Environmental Microbiology 57:2016-2020
  9. Hassan AN, Franka JF, EL & Soda M. 2003. Observation of bacterial exopolysaccharide in dairy products using cryo-scanning electron microscopy. International Dairy Journal 13:755-762
  10. Hosono A, Lee J, Ametani A, Natsume M, Hirayama M, Adachi T & Kaminogawa S. 1997. Characterization of a water-soluble polysaccharide fraction with inmunopotentiating activity from Bifidobacterium adolescentis M101-4. Bioscience, Biotechnology and Biochemistry 61:312-316
  11. Ikeda S and Shisido Y. 2005. Atomic force studies on heat-induced gelation of curdlan. Journal of Agricultural and Food Chemistry 53(3):786-791
  12. Kachlany SC, Levery SB, Kim JS, Reuhs BL, Lion LW & Andghiorse WC. 2001. Structure and carbohydrate analysis of the exopolysaccharide capsule of Pseudomonas putida G7. Environmental Microbiology 12:774-784
  13. Knoshaug EP, Ahlgren JA & Trempy JE. 2000. Growth associated exopolysaccharide expression in Lactococcus lactis subspecies cremoris Ropy352. Journal of Dairy Science 83(4):633-640
  14. Kumar MA, Anandapandian KTK & Parthiban K. 2011. Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium. Brazilian Archives of Biology and Technology 54(2):259-265
  15. Kumar CG, Joo HS, Choi JW, Koo YM & Chang CS. 2004. Purification and characterization of an extracellular polysaccharide from haloalkalophilic Bacillus sp. 1-450. Enzyme and Microbial Technology 34(7):673-681
  16. Liu C, Lin Q, Gao Y, YE L, Xing Y & Xi T. 2007, Characterization and antitumor activity of polysaccharide from Strongylo centrotus nudus eggs. Carbohydrate Polymers 67(3):313- 318
  17. Madiedo PR and Gavila'N CG. 2005. Invited Review: Methods for the screening, isolation, and characterization of exopolysaccharides produced by lactic acid bacteria. Journal Dairy Science 88(3):843-856
  18. Madigan MT, Martinko JM & Parker J. 1997. Brock Biology of Microorganisms (8th edn). Prentice Hall International Ltd., London, UK
  19. Mao R, Tang J & Swanson BG. 2001. Water holding capacity and microstructure of gellan gums. Carbohydrate Polymers 46:365-371
  20. Miller JN and Whistler RL. 1996. Carbohydrates. In Fennema OR (ed) Food chemistry (3rd edn). Marcel Dekker, New York
  21. Paulo EM, Vasconselos MP, Oliveira IS, Affe HM, De Jesus Nascimento R, de Melo IS, Roque MR & de Assis SA. 2012. An alternative method for screening
  22. lactic acid bacteria for the production of exopolysaccharides with rapid confirmation. Food Science and Technology 32(4):710-714 
  23. Roberts CM, Fett WF, Osman SF, Wijey C, O'connor JV & Hoover DG. 1995. Exopolysaccharide production by Bifidobacterium longum BB-79. Journal of Applied Bacteriology 78:463-468
  24. Sajna KV, Sukumaran RK, Gottumukkala LD, Jayamurthy H, Dhar KS & Pandey A. 2013. Studies on structural and physical characteristics of a novel exopolysaccharide from Pseudozyma sp. NII 08165. International Journal of Biological Macromolecules 59:84-89
  25. Sanchez JI, Martinez B, Guillen G, Jimenez-Diaz R & Rodriguez A. 2006. Culture conditions determine the balance between two different exopolysaccharides produced by Lactobacillus pentosus LPS26. Applied and Environmental Microbiology 72(12):7495- 7502
  26. Sutherland IW. 1990. Biotechnology of Microbial Exopolysaccharides. Cambridge University Press, Cambridge, United Kingdom
  27. Tallon R, Bressollier P & Urdaci MC. 2003. Isolation and characterization of two exopolysaccharides produced by Lactobacillus plantarum EP 56. Research in Microbiology 154(10):705-712
  28. Tao F, Biao GZ, Yu JZ & Ning ZH, 2008. Isolation and characterization of an acidic polysaccharide from Mesona Blumes gum. Carbohydrate Polymers 71(2):159-169
  29. Vijayabaskar P, Babinastarlin S, Shankar T, Sivakumar T & Anandapandian KTK. 2011. Quantification and characterization of exopolysaccharides from Bacillus subtilis. MTCC 121. Advances in Biological Research 5(2):71-76
  30. Wang Y, Li C, Liu P, Ahmed Z, Xiao P & Bai X. 2010. Physical characterization of exopolysaccharide produced by Lactobacillus plantarum KF5 isolated from Tibet kefir. Carbohydrate Polymers 82(3):895-903
  31. Wang Y, Ahmed Z, Feng W, Li C & Song S. 2008. Physicochemical properties of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefir. International Journal Biological Macromolecules 43(3):283-288
  32. Wu J, Zhang Y, Wang L, Xie B, Wang H & Deng S. 2006. Visualization of single and aggregated hulless oat (Avena nuda L.) (1-3), (1-4)-β-D-glucan molecules by atomic force microscopy and confocal scanning laser microscopy. Journal of Agricultural and Food Chemistry 54(3):925-934
  33. Yang Z, Li S, Zhang X, Zeng X, Li D, Zhao Y & Zhang J. 2010. Capsular and slime- polysaccharide production by Lactobacillus rhamnosus JAAS8 isolated from Chinese sauerkraut: Potential application in fermented milk products. Journal of Bioscience and Bioengineering 110(1):53-57

Tools


Copyright © 2025  KITE Digital Educational Solutions |  Exclusively distributed by CE-Logic