HomeAnnals of Tropical Researchvol. 41 no. 1 (2019)

Extrapolating The Bypass Potential Of Treated Madre De Agua (Trichanthera Gigantea Nees) Leaf Meal As Protein Source In Rumen-Fistulated Brahman Cattle

Angelo Francis F. Atole | Lolito C. Bestil

 

Abstract:

This study assessed the effectiveness of formaldehyde, heat, and tannic acid treatments of leaf meal (MALM) in reducing the degradation of dry matter (DMD) and crude protein (CPD) in the rumen for increased supply of bypass protein at the intestinal level. The experiment utilized a rumen-fistulated Brahman bull fed with chopped Napier grass soilage (basal diet) and MALM (test diet) at 70:30 basal:test diet ratio. Nylon bags (porosity of ±53μm) containing the treated MALM were incubated in the rumen for 24, 48 and 72h following the “sequential addition” method for in situ degradation measurement. Results showed that DMD of MALM was significantly reduced by formaldehyde treatment after 24h and 48h period of incubation than the untreated. This was followed by tannic acid treatment, though the reduction was significant only after 48h incubation than the untreated. At 72h period of incubation, DMD remained to be significantly lowest with formaldehyde treatment than the untreated or heat and tannic acid treatments. A similar pattern of differences in DMD rate (%/h) was observed as that of DMD (%). The CPD of MALM was also significantly reduced with formaldehyde treatment after 24h incubation than the untreated. At 48h incubation, all treatment methods showed significant protection of protein in MALM over that of the untreated. At 72h, formaldehyde and heat treatments significantly reduced the CPD of MALM compared to the untreated. A similar pattern of differences in CPD rate (%/h) was observed as that of CPD (%). Among treatment methods, the use of formaldehyde is the best, followed by tannic acid treatment, in achieving rumen bypass and promoting greater amino acid supply at the intestinal level.



References:

  1. Angel JE. 1988. Avances en la evaluacion de recursos nutricionales tropicales en Colombia. In Reporte de Investigacion 1(1) (pp26). CIPAV, Cali, Colombia
  2. Atole AFF and Bestil LC. 2014. Extrapolating bypass protein potential of treated soybean meal by in situ degradation in rumen-fistulated Brahman cattle. Annals of Tropical Research 36(1):50-62
  3. Bateman HG, Clark JH & Murphy MR. 2005. Development of a system to predict feed protein flow to the small intestine of cattle. Journal of Dairy Science 88:282-295
  4. Broderick GA and Craig WM. 1980. Effect of heat treatment on ruminal degradation and escape and intestinal digestibility of cottonseed meal. The Journal of Nutrition 110(12):2381-2389
  5. Castro SB, Philip LE, Lappiere H, Jardon PW & Berthiaume R. 2007. Ruminal degradability and intestinal digestibility of protein and amino acids in treated soybean meal products. Journal of Dairy Science 90(2):810-822
  6. Frutos P, Hervas G, Giráldez FJ & Mantecon AR. 2004. Review. Tannins and ruminant nutrition. Spanish Journal of Agricultural Research 2(2):191-202
  7. Gareab FH. 1986. Digestive physiology and ruminant Nutrition. Part2. College of Agriculture-University of Basrah
  8. Glimp HA, Karr MR, Little CO, Woolfolk PG, Mitchell JR GE & Hudson LW. 1967. Effect of reducing soybean protein solubility by dry heat on the protein utilization of young lambs. Journal of Animal Science 26(4): 858-861
  9. Hatfield EE, Driedger A, Garrigus US, Lamb PE & Doane BB. 1969. Effect of treating soybean meal with tannins. Beef Day Report. University of Illinois, Urbana-Champaign
  10. Hemsley JA, Hogan JP & Weston TH. 1970. Protection of forage protein from ruminal degradation. Proceedings of the XI International Grassland Congress (pp703-6). Australia
  11. Hiristov AN, Etter RP, Ropp JK & Granden KL. 2004. Effect of dietary crude protein level and degradability on ruminal fermentation and nitrogen utilization in lactating dairy cow. Journal of Animal Science 82(11):3219-3229
  12. Kearl LC. 1982. Nutrient requirements of ruminants in developing countries. International Feedstuffs Institute, Utah Agricultural Experiment Station, Utah University, Logan, Utah
  13. McMahon LR, McAllister TA, Berg BP, Majak W, Acharya SN, Popp JD, Coulman BE, Wang Y & Cheng KJ. 2000. A review of the effects of forage condensed tannins on ruminal fermentation and bloat in grazing cattle. Canadian Journal of Plant Science 80(3):469-485
  14. Nagel SA and Broderick GA. 1992. Effect of formic acid or formaldehyde treatment of alfalfa silage on nutrient utilization by dairy cows. Journal of Dairy Science 75(1):140-154
  15. Nishimuta JF, Ely DG & Boling JA. 1974. Ruminal bypass of dietary soybean protein treated with heat, formalin and tannic acid. Journal of Animal Science 35(5):952-957
  16. Osuji PO, Nsahlai IV & Khalili H. 1993. Feed evaluation. ILCA manual 5. ILCA (International Livestock Centre for Africa), Addis Ababa, Ethiopia (pp40). Roughages. ILRI experience. Animal Feed Science and Technology 69(1-3):27-38
  17. Playne MJ, Khumnualthong W & Echevarria MG.1978. Factors affecting the digestion of oesophageal fistula samples and hay samples in nylon bags in the rumen of cattle. The Journal of Agricultural Science 90(1):193-204
  18. Preston TR. 1986. Better utilization of crop residues and by-products in animal feeding: Research quidelines 2. A practical manual for research workers. Food and Agriculture Organization Animal Production and Health Paper 50 (pp2-10)
  19. Rosales M, Preston TR & Vargas JE. 1992. Advances in the characterization of non conventional resources with potential use in animal production. British Society of Animal Production. Animal Production in Developing Countries 16:228-229
  20. Rosales M. 1996. In vitro assessment of the nutritive value of mixtures of leaves from tropical fodder trees (PhD dissertation). University of Oxford
  21. Schofield P, Mbugua DM & Pell AN. 2001. Analysis of condensed tannins: a review. Animal Feed Science and Technology 91(1-2):21-40
  22. Shamoon SA, Saleh MN & Abbo NY. 2009. Effects of different levels of protein. treated with formaldehyde on nutrients digestibility and some rumen and blood parameters in Awassi sheep. Iraqi Journal of Veterinary Sciences 23(2):169-173
  23. Snyman LD. 1993. Effect of exposure on the chemical composition and in vitro dry matter digestibility of untreated and formalin treated green chop. Animal Feed Science and Technology 44(3-4):275-280
  24. Tagari H, Pena F & Satter LD. 1986. Protein degradation by rumen microbes of heat- treated whole cottonseed. Journal of Animal Science 62(6):1732-1736
  25. Woolford MK. 1975. Microbiological screening of food preservatives, cold sterilants and specific antimicrobial agents as potential silage additives. Journal of the Science of Food and Agriculture 26(2):229-237