Biocontrol potential of Bacillus thuringiensis var. aizawai and Metarhizium anisopliae SPW isolate against insect pests of pechay and lettuce grown under protected and open field cultivation

Reny  G. Gerona,; Bernardita  I. Lauro,

Biocontrol potential of Bacillus thuringiensis var. aizawai and Metarhizium anisopliae SPW isolate against insect pests of pechay and lettuce grown under protected and open field cultivation

Abstract:

Insect pests are one of the constraints in lettuce and pechay production. Although synthetic chemical insecticides are widely used, organic growers are interested in using alternative options which have no toxic residues. This study aimed to evaluate the biocontrol potential of Bacillus thuringiensis var. aizawai (Bta) and Metarhizium anisopliae (Ma) SPW isolate against insect pests of pechay and lettuce grown under protected and open field cultivation. Weekly spraying of each treatment was done using the recommended rates (RR) of application: Ma at 3L spore suspension 13L water (1x10 spore concentration); Bta at 20g 16L per per 8 water; cypermethrin 5EC at 30m 16L water. Insect infestation was monitored L per weekly based on insect count and damage ratings. Yields were recorded at harvest. Results showed that application of either Bta, Ma or cypermethrin significantly reduced populations and damage Spodoptera litura of Fabr. Plutella xylostella and L. Pechay plants applied with Bta and showed higher yields than cypermethrin in both types of cultivation. These findings suggested that Bta and SPW isolate were effective against S. litura and P xylostella and can be used as an alternative non-chemical option for their management.

References:

Cruda KMB. 2012. Efficacy of Metarrhizium anisopliae (Metsch.) Sorokin and Bacillus thuringiensis Berliner against eggplant fruit and shoot borer Leucinodes orbonalis Guenee (Lepidoptera: Pyralidae) (Undergraduate thesis) (pp36). Visayas State University, Visca, Baybay City, Leyte
Dela Cruz CS, Gapasin RM, Gerona RG, Almeroda BB, Tisado MSP & Agres MBL. 2013. Biological control of jackfruit fruit borer using a friendly fungus, Metarhizium anisopliae SPW isolate. PCAARRD Info Bull 34(11)
Dutta S. 2012. An ecofriendly approach for pest control. World journal of pharmacy and pharmaceutical sciences. SJIF Impact Factor 5.2104(6):250-265. Review Article ISSN 2278-4357. Deptartment of Pharmacology, NSHM College of Pharmaceutical Technology, NSHM Knowledge Campus, Kolkata Group of Institutions, 124(60). B.L.Saha Road, Kolkata-700053
Ekesi S, Maniania NK, Ampong-Nyarko K & Onu I. 1999. Effect of Intercropping Cowpea with Maize on the Performance of Metarhizium anisopliae Against Megalurothrips sjostedti (Thysanoptera: Thripidae) and Predators. Environmental Entomology 28(6):1154-1161. doi:http://dx.doi.org/10/1093/ee/28.6.11541154-116
Freed S, Ahmad Saleem M. Khan MB & Naeem M. 2012. Prevalence and Effectiveness of Metarhizium anisopliae Against Spodoptera exigua (Lepidoptera: Noctuidae) in Southern Punjab, Pakistan University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan. Pakistan Journal of Zoology 44(3):753-758
Gonzaga ZC. 2012. Low-cost Protected Cultivation: Enhancing Year-round Production of High-value Vegetables in the Philippines (pp123-137). In Oakshott J and Hall D (eds) Smallholder HOPES-horticulture, people and soil. 2013. Referred proceedings of the ACIAR-PCAARRD Southern Philippines Fruits and Vegetables Program Meeting, 3 July 2012, Cebu, Philippines. ACIAR Proceedings 139 (pp298), Australian Centre for International Agricultural Research: Canberra. (ISBN 978-1-922137-42-5 (print)); (ISBN 978-1-922137-43-2 (online)
Loc NT and Chi VTB. 2007. Biocontrol and potential of Metarhizium anisopliae and Beauveria bassiana against diamondback moth, Plutella xylostella. Omonrice 15:86-39
Mau R, Dunbar DM, Gusukuma-Minuto L & Shimabuku RS. 2016. Management of diamondback moth with emamectin benzoate and Bacillus thuringiensis subsp. aizawai insecticides. Retrieved: June7,2016. http://web.entomology. cornell.edu/shelton/diamondbackmoth/pdf/1996papers/1996DBM29.pdf
Moon San AWK and Hue SM. 2017. Mode of infection of Metarhizium spp. fungus and their potential as biological control agents. Journal of Fungi 3:1-30
Oatman ER and Platner GR. 1972. An ecological study of lepidopterous pests affecting lettuce on Coastal Southern California. Environmental Entomology 1:202-204
Panyasiri C, Attathom T & Poehling HM. 2007. Pathogenicity of entomopathogenic fungi- potential candidates to control insect pest on tomato under protected cultivation in Thailand. Journal of Plant Disease and Protection 114(6):278-287
Polanczyk A, Pires da Silva RF & Fiuza LM. 2000. Effectiveness of Bacillus thuringiensis strains against Spodoptera frugiperda (lepidoptera: noctuidae). http://dx.doi.org/10.1590/S1517-83822000000300003. Brazilian Journal of Microbiology 31 (3)
Sanahuja G, Banakar R, Twyman RM, Capell T & Christou P. 2011. Bacillus thuringiensis: A century of research, development and commercial applications. Plant Biotechnology Journal 9:283-300
Tibao Marlon Y. 2012. Insect Pests Succession of Organically Grown Pechay (Brassica chinensis Linnaeus) (Undergraduate thesis). Benguet State University, La Trinidad, Benguet, Philippines
Ujjan AA and Shahzad S. 2012. Use of entomopathogenic fungi for the control of mustard aphid (Lipaphic erysimi) on canola (Brassica napus L.). Pakistan Journal of Botany 44(6):208-2086
Vega FE, Goettel MS, Blackwell M, Chandler D, Jackson MA, Keller S, Koike M, Maniania NK, Monzón A, Ownley BH, Pell JK, Rangel DEN & Roy HE (2009) Fungal entornopathogens: New insights on their ecology. Fungal Ecology 2:149-159
Wannar R, Zhihong X & Haixin Y. 2012. Helicoverpa armigera (Lepidoptera: Noctuidae) and its contact toxicity to pupae and adults of parasitoid Microplitis mediator (Hymenoptera: Braconidae). Acra Entomological Sinica 55(8):941-949. Retrieved: June7,2016: http://www.insect.org.cn/EN/volumn/current.shtml