Home⇛Annals of Tropical Research⇛vol. 43 no. 1 (2021)

Heavy Metal Tolerance Of Filamentous Fungi From The Sediments Of Visayas State University Wastewater Pond

Richie Mar M. Eliseo | Jayzon G. Bitacura

 

Abstract:

The ability to tolerate high concentrations of heavy metals is one important characteristic of organisms that can be used for bioremediation. In this study, the heavy metal tolerance of filamentous fungi isolated from the VSU wastewater settlement pond was investigated. Specifically, the research was done to determine the Cd, Cu, Fe and Zn in the sediments of the pond, isolate and identify filamentous fungi from these sediments, identify the most tolerant isolate, and determine the minimum inhibitory concentration of heavy metals to the identified isolate. Isolation of filamentous fungi from a composite of eight sediment samples was done through serial dilution and plating using Potato Dextrose Agar (PDA). Isolates were then purified using Sabouraud Dextrose Agar (SDA). Colony and microscopic characteristics of the isolates were used to identify the isolates to genus level only. The tolerance of the isolates to Cd, Cu, Fe, and Zn were then compared through analysis of their tolerance index based on the colony extension radius. The Minimum Inhibitory Concentrations (MIC) of these heavy metals were obtained for the isolate with the highest tolerance index. The Cd, Cu, Fe, and Zn contents of the composite sediment sample were <0.0002mg kg , 0.0203mg kg , 7.419mg kg , and -1 -1 -1 0.106mg kg , respectively. Three filamentous fungi were successfully isolated -1 from the sediments and were identified as Rhizopus sp., Mucor sp. and Trichoderma sp. Among these isolates, Rhizopus sp. was the most tolerant to all the heavy metals tested. Average range of MIC values of Cd, Cu, Fe and Zn to Rhizopus sp. were determined at 5mM<MIC≤6.5mM, 10mM<MIC≤15mM, 30mM<MIC≤35mM, and 25mM<MIC≤30mM, respectively. These results showed that the Rhizopus sp. isolated is the most potential candidate in bioremediating heavy metal contaminated areas.



References:

  1. Ahmad I, Zafarn S & Ahmad F. 2005. Heavy metal biosorption potential of Aspergillus and Rhizopus sp. Isolated from wastewater treated soil. Journal of Applied Sciences and Environmental Management 9(1):123-126
  2. Anand P, Isar J, Saran S & Saxena R. 2006. Bioaccumulation of copper by Trichoderma viride. Bioresource Technology 97(8):1018-1025
  3. Balsalobre L, De Siloniz MI, Valderrama MJ, Benito T, Larrea MT & Peinado JM. 2003. Occurrence of yeasts in municipal wastes and their behavior in presence of cadmium, copper and zinc. Journal of Basic Microbiology 43(3):185-193
  4. Bhattacharya SE. 2011. Mycoremediation of Congo red dye of filamentous fungi. Brazilian Journal of Microbiology 42(4):1526-1536
  5. Brand A. 2012. Hyphal growth on human fungal pathogens and its role in virulence. International Journal of Microbiology 2012:517-529
  6. Brandolini V, Tedeschi P, Capece A, Maietti A, Mazzotta D, Salzano G, Paparella A & Romano P. 2002. Saccharomyces cerevisiae wine strains differing in copper resistance exhibiting capability to reduce copper content in wine. World Journal of Microbiology and Biotechnology 18(6):499-503
  7. Canadian Council of Ministers of the Environment (CCME). 1992. Canadian water quality guidelines, CCME, Inland Water Directorate, Ottawa, Ontario. In Drotse R (ed) Theory and Practice of Water and Wastewater Treatment (pp790). John Wiley and Sons, Inc
  8. Dacera DP, Cortes DS, David FC, Miraflor KS, Plimaco PT & Bitacura JG. 2016. Filamentous fungi from coastal sediments as novel indicators of long-term heavy metal contamination (Unpublished research). VSU Laboratory High School
  9. Darlington AB and Rauser WE. 1998. Cadmium alters the growth of the mycorrhizal fungus Paxillus involutus: a new growth model accounts for changes in branching. Canadian Journal of Botany 66(2):225-229
  10. Ezzouhri L. 2009. Heavy metal tolerance of filamentous fungi Isolated from polluted sites in Tangier, Morocco. African Journal of Microbiology Research 3(2):35-48
  11. Gadd G. 1993. Interactions of fungi with toxic metals. New Phytologist 124:25-60
  12. Harms H. 2011. Untapped potential exploiting fungi in bioremediation of hazardous chemicals. Nature Reviews Microbiology 9:177-192
  13. Jarup L. 2003. Hazards of heavy metal contamination. British Medical Bulletin 68(1):167- 182
  14. Kensa MV. 2011. Bioremediation an overview. Journal of Industrial Pollution Control 27(2):161-168
  15. Manguilimotan LC and Bitacura JG. 2018. Biosorption of cadmium by filamentous fungi isolated from coastal water and sediments. Journal of Toxicology 2018:1-6 
  16. New Jersey Department of Health. 1999. Right to know hazardous substance fact sheet. https://web.doh.state.nj.us/rtkhsfs/indexfs.aspx
  17. Oladipo OG, Awotoye 00, Olayinka A, Bezuidenhout CC & Maboeta MS. 2018. Heavy metal tolerance traits of filamentous fungi isolated from gold and gemstone mining sites. Brazilian Journal in Microbiology 49(1):29-37
  18. Pal TK, Bhattacharyya S & Basumajumdar A. 2010. Cellular distribution of bioaccumulated toxic heavy metals in Aspergillus niger and Rhizopus arrhizus. International Journal of Pharma and Bio Sciences 1(2):1-6
  19. Rhodes C. 2013. Application of bioremediation and phytoremediation. Science Progress 96(4):417-427
  20. Rholand L. 2013. Merritt’s neurology. Lippincott Williams & Wilkins (LWW)
  21. Singh R. 2011. Heavy metals and living systems: an overview. Indian Journal of Pharmacology 43(3):246-253
  22. Slaninova A. 2014. Fish kill caused by aluminum and iron contamination in natural pond used for fish rearing: a case report. Veterinární Medicína 59(11):573-581
  23. Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. 2012. Heavy metal toxicity and the environment. In Luch A (ed) Molecular, Clinical and Environmental Toxicology. Experientia Supplementum 101:133-164. Springer, Basel
  24. Tsekova K and Galabova D. 2003. Phosphatase production and activity in copper (II) accumulating Rhizopus delemar. Enzyme and Microbial Technology 33(7):926-931
  25. Valenzona H. 2012. Cadmium, lead and copper analysis of water from the Visayas State University wasterwater lagoon using flame atomic absorption Spectrophotometry (Undergraduate thesis). Visayas State University, Philippines
  26. Watkinson S, Boddy L & Money N. 2015. The fungi (3rd edn). Academic Press
  27. Zafar S. 2007. Metal toleramce and biosorption potential of filamentous fungi isolated from metal contaminated agriculture soil. Bioresource Technology 98(13):2557-2561

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