HomeAnnals of Tropical Researchvol. 28 no. 1 (2006)

Biochemistry of postharvest spoilage of sweetpotato (Ipomoea batatas L.) 2. Comparison of cellulolytic enzyme production in cultures and fungi-infected sweetpotato tubers

Ramesh C. Ray

 

Abstract:

Ray, R. C. 2006.Biochemistry of postharvest spoilage of sweet potato (/pomoea batatas L.). 2. Comparison of cellulolytic enzyme production in cultures and fungi-infected sweet pota:: tubers. Ann. Trop. Res. 28(1 ):48-57 The study was conducted to detennine the production in vitro and in vivo ef cellulases by Botrydiplodia theobromae and Rhizopus oryzae. Isolates of these organisms were obtained from the postharvest decay of sweetpotato tubers. Results revealed that B. theobromae and R. oryzae which were isolated fro-postharvest spoilage of sweetpotato tubers produced endo- -1,4-glucanase and exo-'-l,4-glucanase in culture and in fungi-infected tissues of sweet potato tubers. The optimum: temperature and pH for cellulase synthesis and activity were 30°C and pH 6.5, respective!



References:

  1. ADISA, V. A. and A. O. FAJOLA. 1983. Cellulolytic enzymes associated with the fruit rots of Citrus sinensis caused by Aspergillus aculeatus and Botryodiplodia theobromae. Mycopathol. 82:23-27.
  2. AMADIOHA A. C. 1993. Production of cellulytic enzymes by Rhizopus oryzae in culture and Rhizopus-infected tissues of potato tubers. Mycologia. 85:574-578.
  3. BAGGA, P. S. and D. K. SANDHU. 1987. Cellulase formation by Aspergillus nidulans. J Ferment. Technol. 65:635-642.
  4. FAMUREWA O. and P. O. OLUTIOLA. 1991. Comparison of growth and cellulolytic enzyme production in Aspergillus chevalieri and Penicillium steckii from mouldy cocoa beans. Folia Microbiol. 36:347-352.
  5. FESSEHATZION B. and P. O. OLUTIOLA. 1987. A proteolytic enzyme associated with the infection of cocoa beans by Botryodiplodia theobromae. FEMS Microbiol. Lett. 43:67-70.
  6. LAHOZ, R., F. REYES and M. I. PEREZ LEBIC. 1976. Lytic enzymes in the autolysis of filamentous fungi. Mycopathol. 60:45-49.
  7. MAHADEVAN A. and R. SRIDHAR. 1998. Methods in Physiological Plant Pathology Sivakami Publicaitons, Madras, 326 pp.
  8. MANDELS, A. and D. STEINBERG. 1976. Recent advances in cellulase technology.J Ferment Technol. 54:267-286.
  9. THAR WAL, P. D., H. N. GOUR and S. AGFARWAL. 1991. Effects of different factors the production of cellulase by Curvularia lunata. Folia Microbiol. 36:357-360
  10. C. and R. S. MISRA. 1995. Spoilage of sweetpotato tubers in tropics. I. Microorganisms Associated. Adv. Hort. Sci. 159-163.
  11. C. and E. PUNITHALINGAM. 1996. Spoilage of sweetpotato tubers in tropics II. Java black rot by Botrydiplodia theobromae. Pat. Growth studies and mode of infection. Adv. Hort. Sci. 10:151-157.
  12. RC., R. S. MISRA and P. K. GHOSH. 1997. Spoilage of Sweetpotato tubers in pics. III. Soft rot by Rhizopus oryzae L. Adv. Hort. Sci. 19:103-107.
  13. RESEE. T. and H. E. LEVINSON. 1952. A comparative study of breakdown of cellulose by microorganisms. Physiol. Plant. 5:345-350.
  14. K. F. 1976. Biotrophic parasites in culture. 1. Growth of biotrophic parasites in menic culture. In: Physiological Plant Pathology (R. Heitefuss and R.H. Williams, eds.). Springer Verlag, New York, pp. 719-741.s
  15. MINER, B. F. 1986. Postharvest handling, practices and postharvest diseases of fruits. Plant Diseases. 66:357-364.
  16. LLAREAL, R. L. 1982. Sweetpotato in tropics: Progress and Problems.Proc. 1st Intern. Symp. on Sweetpotato. Asian Veg. Res. Dev. Centre. Taiwan, China. pp. 3-16.
  17. WOOD.R. K. S. 1960. Pectic and cellulolytic enzymes in plant diseases. Ann. Trop. Rev. Plant. Physiol. 11:299-322.