Screening of blue crab (Portunus pelagicus L.) eyestalk extracts for growth inhibition of Pectobacterium carotovorum subsp. carotovorum (Hauber et al. 1998) (Erwinia carotovora) Jones (Holland) and Ralstonia solanacearum (Yabuuchi et al. 1995)

Julissah  C. Evangelio,; Bernadita  P. Germano,

Screening of blue crab (Portunus pelagicus L.) eyestalk extracts for growth inhibition of Pectobacterium carotovorum subsp. carotovorum (Hauber et al. 1998) (Erwinia carotovora) Jones (Holland) and Ralstonia solanacearum (Yabuuchi et al. 1995)

Julissah C Evangelio | Bernadita P. Germano

Abstract:

Evangelic, J.C. and BP. Germano. 2006. Screening of blue crab (Portunus pelagicus L.) eyestat· extracts for growth inhibition of Pectobacteriufn carotovorum subsp. carotovorum (Haub&' et a{ 1998) ( Erwinia carotovora) Jones (Holland) and Ralstonia solanacearum (Yabuucr et al. 1995) (Pseudomonas solanacearum) E. F. Smith. Ann. Trap. Res. 28(2):92-110. Cmstacean eyestalks are knov,rn to contain neurosecretory honnones tha; inhibit molting, growth and gonadal development. Along this framework. this smdy was conducted to: screen crude cyestalk extracts frorn the blue crab Por!/11111.1 pelagic us for possible growth inhibition on t.'O bacterial pathogens Ralston/a so!w,acean,m and Pectobacterium carotovorum subsp. carotovornm, and determine effective concentrations of the crnde extract against the pathogens. The screening was done through bioassay using the top agar inoculation technique with 70% ethanol as the extracting solvent a11d streptomycin as the positive control. Different concentrations of blue crab extract and streptomycin were obtained using serial dilution.

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