Effect Of Different Factors On Germination And Tube Growth Of Solanum Sisymbrifolium Lam. Pollen
K M. Kuruvilla | Y S. Chauhan | G R. Das | J S. Kharbteng
Abstract:
Both germination and tube growth of Solanum sisymbrifolium Lam. pollen quire an optimum temperature of 25°C and a growth medium containing 100 ppm boric acid, 10% sucrose and 0.6% Bacto-agar. Pollen germination was stimulated by 5 and 10 ppm GAJ, 1-10 ppm kinetin, 1 ppm ethylene, and 1 and 5 ppm ABA. Higher concentrations of these growth hormones reduced pollen germinability. Pollen tube elongation was stimulated by 10 ppm GA3, 1-10 ppm kinetin, 1 ppm ethylene and 1-10 ppm ABA. IAA at 5 to 25 ppm inhibited both pollen germination and tube growth. The degree of inhibition increased with higher concentrations of IAA.
References:
- BALAKRISHNAN, N.P. 1983. Flora of Jowai and vicinity, Meghalaya vol. 2. 331 pp.
- BASLER, E. 1974. Abscissic acid and gibberellic acid as factors in the translocation of auxin. Plant and Cell Physiol. 15: 351-360.
- BREWBAKER, J.L. and KWACK, B.H. 1963. The essential role of calcium ion in pollen germination and pollen tube growth. Amer. J. Bot. 50: 859-865.
- BREWBAKER, J.L. and MAJUMDER, S.K. 1961. Cultural studies of pollen population effect and the self-incompatibility inhibition. Amer. J. Bot. 48: 457-464.
- BUCHANAN, D.W. and BRIGGS, R.B. 1969. Peach fruit abscission and pollen germination as influenced by ethylene and 2-chloroethane phosphonic acid. Proc. Amer. Soc. Hort. Sci. 94: 327-329.
- CHAUHAN, Y.S. and KHARBTENG, J.S. 1986. ‘Ethrel’ effect on pollen germination and pollen tube growth of alkaloid yielding solanums. Geobios 13: 231-232.
- JOHRI, B.M. and VASIL, I.K. 1961. Physiology of pollen. Bot. Rev. 27: 325-381.
- JOHRI, B.M., SASTRI, I.C. and SHIVANNA, K.R. 1977. Pollen viability, storage and germina-tion. In: Advances in Pollen Spore Research. P.K.K. Nair (ed.). Today and Tomorrow’s Printers and Publishers, New Delhi. vol. II. pp. 1-20.
- KAUL, B.L. and ZUTSHI, U. 1977. Cultivation of Solanum khasianum Cl. for steroids: Problems and promises. In: Cultivation and Utilization of Medicinal and Aromatic Plants. C.K. Atal and B.M. Kapur (eds.). C.S.I.R. Regional Research Laboratory, Jammu Tawi. pp. 23-31.
- LEOPOLD, A.C. and KRIEDEMANN, P.E. 1975. Plant Growth and Development. Tata McGraw Hill Publishing Co. Ltd., New Delhi. 545 pp.
- LINSKENS, R.F. and KROH, M. 1970. Regulation of pollen tube growth. In: Current Topics in Developmental Biology. A.A. Moscana and A. Monroy (eds.). Academic Press, London. vol. 5. pp. 89-113.
- MALIK, C.P. and CHHABRA, N. 1976. Hormonal regulation of pollen germination and pollen tube elongation in Arachis hypogea Reitz. Proc. Indian Acad. Sci. 84: 101-108.
- MASCARENHAS, J.P. and MACHLIS, L. 1964. Chemotropic response of the pollen of Antir-rhinum majus to calcium. Plant Physiol. 39: 70-77.
- MCLEOD, Κ.Α. 1975. The control of growth of tomato pollen. Ann. Bot. 39: 591-596.
- MEHAN, M. and MALIK, M. 1975. Studies on the effect of different growth regulators on the elongation of pollen tube in Calotropis procera. J. Palynology 11: 71-77.
- MUKHERJEE, S.K. and DAS, P.K. 1964. Palynology in horticuture and plant breeding. In: Recent Advances in Palynology. P.K.K. Nair (ed.). National Botanical Gardens, Luck-now. pp. 305-326.
- O’KELLEY, J.C. 1957. Boron effects on growth, oxygen uptake and sugar absorption by germinat-ing pollen. Amer. J. Bot. 44: 239-244.
- PANDEYA, S.C, SARATBABU, G.V. and BHATT, A.B. 1981. Dynamics of solasodine ac-cumulation in developing berries of Solanum sisymbrifolium. Planta Medica 42(4): 409-411.
- RAVINDRAN, S. and CHAUHAN, Y.S. 1980. Studies in the reproductive biology of alkaloid yielding solanums. I. Temperature, sucrose and boron requirements for pollen germina-tion and pollen tube elongation. J. Palynology 16 (1 & 2): 53-58.
- RAVINDRAN, S. and CHAUHAN, Y.S. 1986. Studies in the reproductive biology of alkaloid yielding solanums. V. In vitro responses of pollen grain of S. khasianum Cl. and S. indicum L. to plant growth hormones. Acta Botanica Indica 14: 130 - 132.
- SASTRI, D.C. 1974. Effect of ethephon on pollen germination. (Abstr.). In: First Indian Palynological Congress. Punjab Univ., Chandigarh, India. pp. 4-5.
- SHIVANNA, K.R. and JOHRI, B.M. 1985. The Angiosperm Pollen Structure and Function. Wiley Eastern Ltd., New Delhi.
- STANLEY, R.G. and LOEWUS, F.A. 1964. Boron and myoinositol in pollen pectin biosynthesis. In: Pollen Physiology and Fertilization. H.F. Linskens (ed.). North-Holland, Amsterdam. pp. 128-139.
- VASIL, I.K. 1964. Effect of boron on pollen germination and pollen tube growth. In: Pollen Physiology and Fertilization. H.F. Linskens (ed.). North-Holland, Amsterdam. pp. 107-119.
- VASIL, I.K. 1974. The histology and physiology of pollen germination and pollen tube growth on the stigma and in the style. In: Fertilization in Higher Plants. H.F. Linskens (ed.). North-Holland, Amsterdam. pp. 105-118.
- VISSER, T. 1955. Germination and storage of pollen. Meded. Landb. Hoogesch (Wageningen) 55:1-68.
- YADAV, V.B. 1980. Effect of some growth regulators on pollen germination and pollen tube growth in Cassia tora L. and C. obtusifolia L. Comp. Physiol. Ecol. 5: 165-168.
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