HomeAnnals of Tropical Researchvol. 8 no. 3 (1986)

Effect Of Pectinase, Cellulase And Nutrient Addition On Fermentation Of Sweet Potato Mashes

Richard N. McArdle | John C. Bouwkamp

 

Abstract:

Addition of either cellulase or pectinase mixture to pre-heated sweet potato mashes at the time of yeast inoculation increased ethanol production up to twice that of untreated mashes, possibly by enhancing the post-heating activity of the native amylase and/or by lowering the viscosity of the mash, thus allowing better mixing and providing more chances for amylase attack on starch granules. With enzyme addition, heating mashes beyond 60 ° C did not increase ethanol yield. However, ethanol production was higher if enzymes were not added. The use of a common yeast nutrient supplement, corn steep, was detrimental to fermentation.



References:

  1. AZHAR, A. 1981. Alcohol fermentation of sweet potato. I. Acid hydroly-sis and factors involved. Biotech. Bioeng. 23:876-879.
  2. AZHAR, A. and HAMDY, M.K. 1981. Alcohol fermentation of sweet potato. Membrane reactor in enzymatic hydrolysis. Biotech. Bioeng 23:1297-1307.
  3. BOUWKAMP, J.C. 1984. The potential of sweet potatoes and their problems. World Crops 36(2):59-62.
  4. CHUA, J.W., FUKUI, N., WAKABAYASHI, Y., YOSHIDA, T. and TAGUCHI, H. 1984. Enzymatic hydrolysis of sweet potato for energy-saving production of ethanol. J. Ferment. Technol. 62:123-130.
  5. DAVIS, P.L. and CHACE, W.G. 1969. Determination of alcohol in citrus juice by gas chromatographic analysis of headspace. HortScience 4:117-119.
  6. HOOVER, M.W. and HARMON, S.J. 1967. Carbohydrate changes in sweet potato flakes made by the enzyme activation method. Food Technol. 21:1529-1532.
  7. JOHNSON, A.H. and PETERSON, M.S. 1974. Encyclopedia of Food Technology. AVI Publ. Co., Westport, CT, U.S.A.
  8. MATSUOKA, H., KOBA, Y. and UEDA, S. 1982. Alcoholic fermenta-tion of sweet potato without cooking. J. Ferment. Technol. 60:599-602.
  9. MCARDLE, R.N. and BOUWKAMP, J.C. 1986. Use of heat treatments for saccharification of sweet potato mashes. J. Food Sci. 51:364-366.
  10. SCOTT, L.E. and MATTHEWS, W.A. 1957. Carbohydrate changes in sweet potatoes during curing and storage. Proc. Amer. Soc. Hort. Sci. 70:407-418.
  11. SISTRUNK, W.A. 1977. Relationship of storage, handling, and cooking method to color, hardcore tissue and carbohydrate composition in sweet potatoes. J. Amer. Soc. Hort. Sci. 102:381-384.
  12. SREEKANTIAH, K.R. and RAO, B.A.S. 1980. Production of ethyl alcohol from tubers. J. Food Sci. Technol. 17:194-195.
  13. SVENDSBY, D., KAKUTANI, K., MATSUMURA, Y., IISUKA, M. and YAMAMOTO, Y. 1981. Ethanol fermentation of uncooked sweet potato with the application of enzymes. J. Ferment. Technol. 59:485-487.
  14. UEDA, S. and KOBA, Y. 1980. Alcohol fermentation of raw starch with-out cooking by using black-koji amylase. J. Ferment. Technol. 58:237-242.