Bioassay-Guided Isolation Of The Antimicrobial Compounds Of Coleus Amboinicus Loureiro (Lamiaceae)

Erlinda  A. Vasquez,; Claus Peter  Zebitz,; William  E. Kraus,; Joseph  Conrad,; Benedikt  Volger,; Belen  M. Rejesus,

Bioassay-Guided Isolation Of The Antimicrobial Compounds Of Coleus Amboinicus Loureiro (Lamiaceae)

Abstract:

The essential oil of the aerial part of Coleus amboinicus showed antibacterial activity against Bacillus subtilis (gram +) and P~·eudomonas fluorescens (gram -) and antifungal activity against Cladosporium cucumerinum. Bioassay-guided fractionation revealed that carvacrol and P-caryophyllene-4,5-oxide were the major contnbutors to the antimicrobial activity of the essential oil. The sesquiterpene a-humulene and monoterpenes a.-pinene, o:- terpinene and cymene present in significant amounts were inactive.

References:

BARRERO, A. F., J. MOLINA, J. E. OLTRA, J. ALTAREJOS, A. BARRAGAN, A. LARAand M. SEGURA. 1995. Stereochemistry of 14-hydroxy-ẞ-caryophyllene and related compounds. Tetrahedron. 51(13):3812-3822.
BOHLMANN, F., R. ZEISBERG, E. KLEIN. 1975. 13C NMR Spektren von Monoterpenen. Org. Mag. Res. 7:426-432.
DEV, S., A. P. S. NARULA and J. S. YADAV. 1982. CRC Handbook of Terpenoids. Vol.1.S. Dev (ed). CRC Press, Inc. Boca raton, FL.
LUND, B. M., G. D. LYON. 1975. Detection of inhibitors of Erwinina carotova and E. herbicola on thin layer chromatograms. J. Chromatography. 110:193-196.
HAMBURGER, M. D. and GA. CORDELL. 1987. A direct autobiography TLC assay for compounds possessing antibacterial activity. J. Nat. Prod. 50, 19-22.
HOMAN, A. I., and A. FUCHS. 1987. Direct autobiography on thin-layer chromatograms as a method for detecting fungitoxic substances. J. Chrom. 5, 327-329.
JANSSEN, A. M., J. J. SCHEFFER, C. BAERHEIM and A. SVENDSEN. 1986. Antimicrobial Screening of Essential Oil - Aspects of the Agar Overlay Technique. In: “Progress in Essential Oil Research” (Brunke, E. J. ed). Walter de Gruyter & Co., Berlin, New York. pp 401-419.
MERRIL, E. U. 1912. A Flora of Manila. Manila Bureau of Printing. 491 p.
MCMURRY, J. E., J. R. MATZ, K. L. KEES. 1987. Synthesis of macrocyclic terpenoids by intramolecular carbonyl coupling: flexibelene and humulene. Tetrahedron 43:5489-5498.
NGUYEN, V. D. and DOAN, T. N. 1990. Medicinal Plants in Vietnam. WHO Regional Publication, Western Pacific Series No. 3 410 pp.
PINO, J., A. RODADO and P. BORGES. 1990. Volatile components in the essential oil of wild oregano (Coleus amboinicus Lour.) Die Nahrung. 34:819-823.
QUISUMBING E. 1975. Medicinal Plants of the Philippines. Katha Publishing Company. Quezon City. 439 р.
RANDRIAMIHARISOA, R., E. M. FAURE and J. P. BIANCHINI. 1986. CARBONM-13 nmr spectra of five sesquiterpenes isolated fromYlang Ylang Essential Oil. Og. Magn. Reson. 24:275-276.
WEEHUIZEN, F. 1918. Sur le phenol des feuilles du Coleus amboinicus Lour. Rec. Trav. Chim. Pays-bays, 37:335-356.
WEIMER, C. 1929. Die Pfanzenstoffe. Vol. 1 Jenna 640. pp