In-vitro Screening for the Antibacterial Activity of Ethanolic Flacourtia jangomas Leaves Extract Against Gram-positive Methicillin -resistant Staphylococcus aureus (MRSA) Strain

Melissa June  Paderog,; Miky Dianne  Dela Fuente,; Ellen Faith  Delgado,; Christine Cymer  Diaz,; Danielle Marie  Dionio,; Nelynmae  Ecullada,; Geverly Mariz  Ellaga,; Angeline  Espartero,; Andrea Grace  Felix,; Arya  Francisco,; Louizel Jamz  Funa,; Marry Joy  Mabanes,; Hazel Mae  Panes,; Allan Marc  Lena,

In-vitro Screening for the Antibacterial Activity of Ethanolic Flacourtia jangomas Leaves Extract Against Gram-positive Methicillin -resistant Staphylococcus aureus (MRSA) Strain

Discipline: Pharmacology

Abstract:

By 2030, the WHO estimates that 5.2 million deaths in the Western Pacific Region will result from drug-resistant bacterial infections, with methicillin-resistant Staphylococcus aureus (MRSA) being a major threat. Flacourtia jangomas, also known as indian plum, is a promising plant-derived source of antibacterial compounds that may serve as an alternative to conventional antibiotics with reduced efficacy due to resistance. With the increasing emergence of antibacterial resistance and the promising potential of plant-derived compounds as antibacterial alternatives, this study aims to shed light on the antibacterial potential of F. jangomas. This study evaluated the antibacterial activity of crude ethanolic extract from F. jangomas leaves against multidrug-resistant S. aureus (HIP10787). Bacterial susceptibility and minimum inhibitory concentration (MIC) were determined using the broth dilution method, while phytochemical screening was conducted to identify active compounds. Results showed that the extract inhibited 54.95% of bacterial growth at 5 μg/mL, compared to 99.53% inhibition by the positive control (0.25 μg/mL vancomycin). The MIC was determined to be 5 μg/mL, with 51.10% inhibition. Phytochemical analysis revealed the presence of alkaloids and terpenoids, which may contribute to the extract’s antibacterial activity, while tannins, flavonoids, and phenolic compounds were absent. This study highlights F. jangomas as a potential source of bioactive compounds against MRSA, supporting further exploration of plant-based antibacterial agents

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