HomeDAVAO RESEARCH JOURNALvol. 11 no. 2 (2016)

Antibacterial Activity of the Aloe vera, Aloe barbadensis Leaf Extracts Against Escherichia coli

Chella May Sison | Marites S. Erespe

Discipline: microbiology and cell science

 

Abstract:

This study aimed to determine the antibacterial activity of the different leaf parts of Aloe barbadensis, specifically aloe vera leaf peel, aloe vera leaf gel, and whole leaf, as well as their medicinal properties. Ethanol was the solvent used in extracting the bioactive compounds present in each leaf part. Each leaf part had an ethanolic concentration of 100% each. The disc diffusion assay was employed to determine the antibacterial activity of Aloe barbadensis. The appearance of zones of inhibition measured the antibacterial effect. Ampicillin served as the positive control, while distilled water served as the negative control. Antimicrobial tests showed that among the three leaf parts, the aloe vera leaf gel was the only extract that inhibited the growth of Escherichia coli with a mean zone of inhibition of 8.56mm. The results suggest that aloe vera gel contains certain bioactive compounds that can suppress the growth of Escherichia coli.



References:

  1. Agaoglu, S. & Suleyman, A. (2009). Investigation of in vitro antimicrobial activity of Aloe vera juice. Journal of Animal and Veterinary Advances, 8 (1), 99-102. https://www.researchgate.net/publication/279896034_Investigation_of_In_vitro_Antimirobial_Activity_of_Aloe_vera_Juice
  2. Agarry, O. O., Olaleye, M. T. & Bello-Michael, C. O. (2005). Comparative antimicrobial activities of aloe vera gel. African Journal of Biotechnology,4 (12), 1413-1414. DOI:10.4314/AJB.V4I12.71436
  3. Ahmed, M. & Hussain, F. (2013). Chemical composition and biochemical activity of Aloe vera (Aloe barbadensis Miller) leaves. International Journal of Chemical and Biochemical Sciences, 3, 29-33.
  4. Akhtar, M., Hai, A., Awais, M. M., Iqbal, Z., Muhammad, F., Ul Haq, A. & Anwar, M. I. (2012). Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens.Veterinary Parasitology, 186, 170-177. DOI:10.1016/j.vpar.2011.11.059
  5. Arunkumar, S. & Muthuselvam, M. (2009). Analysis of phytochemical constituents and antimicrobial activities of aloe vera L. against clinical pathogens. World Journal of Agricultural Sciences, 5 (5), 572-576.
  6. Ashnagar, A. & Naseri, G. (2007). Analysis of the three Penicillin antibiotics (Ampicillin, Amoxicillin and Cloxaciliin) of several Iranian pharmaceutical companies by HPLC. EJournal of Chemistry, 4 (4), 536-545. DOI: https://doi.org/10.1155/2007/480907
  7. Bashir, A., Saeed, B., Mujahid, T. & Jehan, N. (2007). Comparative study of antimicrobial activities of Aloe vera extracts and antibiotics against isolates from skin infections. African Journal of Biotechnology, 10 (19), 3835-3840. https://readpaper.com/paper/2105109858 DOI:10.5897/ajb.9000114
  8. Bhardwaj, R. L. & Nandal, V. (2012). Aloe vera: A valuable wonder plant for food, medicine and cosmetic use- A review. https://www.researchgate.net/publication/287718490_Aloe_vera_A_valuable_wonder_plant_for_food_medicine_and_cosmetic_use_-_a_review.
  9. Bozzi, A., Perrin, C., Austin, S. & Vera, F. A. (2007). Quality and authenticity of commercial aloe vera gel powders. Food Chem, 103 (1), 22-30. https://doi.org/10.1016/j.foodchem.2006.05.061
  10. Cheesebrough, M. (2000). District laboratory practice in tropical countries part 2. Cambridge University Press, 47-57. https://doi.org/10.1017/CBO9780511543470
  11. Choi, S. W., Son, B. W., Son, Y. S., Park, Y. I., Lee, S. K. & Chung, M. H. (2001). The wound healing effect of a glycoprotein fraction isolated from aloe vera. Br. J. Dermatol,145, 535 545 https://doi.org/10.1046/j.1365-2133.2001.04410.x
  12. Chopra, R. N., Nayer, S. L. & Chopra, I. C. (1992). Glossary of indian medicinal plants. Council of Scientific and Industrial Research, 7, 246.
  13. Cock, I. E. (2007). Antimicrobial activity of Aloe barbadensis Miller leaf gel components. The Internet Journal of Microbiology, (1) 2. DOI: https://doi.org/10.5580/15BC
  14. Davis, R. H., Leitner, M. G., Russo, J. M. & Byrne, M. E. (1989). Anti-inflammatory activity of Aloe vera against a spectrum of irritants. Journal of the American Podiatric Medical Association, 79(6), 263–276. DOI: https://doi.org/10.7547/87507315-79-6-263
  15. Dhanabal, S.P., Patel, D.K. & Patel, K. (2012). Phytochemical standardization of aloe vera extract by HPTLC techniques. Journal of Acute Disease, 47-50. DOI: https://doi.org/10.1016/S2221-6189(13)60011-6
  16. Fani, M. & Kohanteb, J. (2012). Inhibitory activity of aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria. Journal of Oral Science, 54 (1), 25-31.DOI: 10.2334/josnusd.54.15
  17. Foster, S. (1999). Aloe vera: The succulent with skin soothing cell protecting properties. Herbs for Health magazine. Health World Online. http://www.healthy.net/library/articles/hfh/aloe.html
  18. Grindlay, D. & Reynolds, T. (1986). “The aloe vera phenomenon: A review of the properties and modern uses of the leaf parenchyma gel”. J. Ethnopharmacol, 16, 117-151. DOI: 10.1016/0378-8741(86)90085-1
  19. Grover, A. Bhandari, B. & Rai, N. (2011). Antimicrobial activity of medicinal plants-Azadirachta indica, A. vuss, Allium cepa L. and Aloe vera L. International Journal of Pharmtech Research, 3 (2), 1059-1065
  20. Hamman, J. H. (2008). Composition and applications of aloe vera leaf gel. Molecules 13: 159 91616. DOI: 10.3390/molecules13081599
  21. Haque, M. Z., Islam, M. B., Jalil, M. A. & Shafique, M.Z. (2014). Proximate analysis of aloe vera leaves. IOSR Journal of Applied Chemistry (IOSR-JAC), 7 (6), 36-40 DOI:10.9790/5736-07613640
  22. Hudzicki, J. (2009). Kirby-Bauer disk diffusion susceptibility test protocol. American Society For Microbiology. https://asm.org/getattachment/2594ce26-bd44-47f6-8287-0657aa9185ad/kirby-bauer-disk-diffusion-susceptibility-test-protocol-pdf.pdf
  23. Hutter, J. A., Salman, M. & Stavinoha, W. B. (1996). Anti-inflammatory C-glucosyl chromone from Aloe barbadensis. Journal of Natural Products, 59 (5), 541-543. DOI: https://doi.org/10.1021/np9601519
  24. Ifeanyi, O. E., Eziokwu, O. G. & Stanley, M. C. (2014). Antimicrobial effects of aloe vera on some human pathogens. International Journal of Current Microbiology and Applied Sciences, 3 (3), 1022-1028.
  25. Iyengar, M. A. (1985). Study of crude drugs. College of Pharmaceutical Sciences, 13, 78.
  26. Iyer, P., Philip, J. & John, S. (2012). Antimicrobial activity of Aloe barbadensis, Daucus carota, Emblica officinalis, Honey and Punica granatum and formulation of a health drink and salad. Malaysian Journal of Microbiology, 8 (3), 141-147.DOI:10.21161/mjm.04012
  27. Japanese Pharmacopoeia. (2007). Society of Japanese pharmacopoeia. Amended Chapters 35.1, 35.2,7. The minister of health, labor, and welfare.
  28. Jeyaseelan, C & Jashothan, J. (2012). In vitro control of Staphylococcus aureus (NCTC 6571) and Escherichia coli (ATCC 25922) By Ricinus communis L. Department of Botany, Faculty of Science, University of Jaffina, Jaffina, Sri Lanka. 2 (10): 717-721. DOI:10.1016/S2221-1691(12)60216-0
  29. Kaper, J., Nataro, J. & Mobley, H. (2004). Pathogenic Escherichia coli. Nature Reviews: Microbiology, 2. DOI: 10.1038/nrmicro818
  30. Karpagam, T. & Devaraj, R. A. (2011). Studies on the efficacy of Aloe vera on antimicrobial activity. International Journal of Research in Ayurveda and Pharmacy, 2, 1286-1289.
  31. Khaing, T. A. (2011). Evaluation of the antifungal and antioxidant activities of the leaf extract of Aloe vera (Aloe barbadensis Miller). In Proceedings of World Academy of Science, Engineering and Technology, 75, 610-612. https://scispace.com/pdf/evaluation-of-the-antifungal-and-antioxidant-activities-of-2gswecff8a.pdf
  32. Kumar, A. P., Joy, J. M., Mohanalakshmi, S. & Prathyusha, S. (2012). Formulation and evaluation of poly herbal hand wash. International Journal of Pharmacy, 2 (2).
  33. Kumari, M. & Gupta, R. P. (2014). Investigation of in vitro antibacterial activity of aloe vera leaves extract on Escheria coli. 53 (2), 151-153. DOI:10.5555/20153127650
  34. Li, X. S., Wang, G. Q., Du, X. D., Cui, B. A., Zhang, S. M. & Shen, J. Z. (2007). Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens. Journal of Veterinary Sciences, 8, 243-247. DOI:10.4142/jvs.2007.8.3.243
  35. Malini, M., Abirami, G., Hemalatha, V. & Annadurai, G. (2010). Antimicrobial activity of ethanolic and aqueous extracts of medicinal plants against waste water pathogens. International Journal of Research in Pure and Applied Microbiology.
  36. Mitscherlich, E. & Marth, E. H. (1984). Microbial survival in the environment-Bacteria and Rickettsiae: Important in human and Animal Health.
  37. O’ Sullivan, J., Bolton, D., Duffy, G., Bagis, C., Tozzoli, R., Wasteson, Y. & Lofdahl, S. (2006). Methods for Detection and molecular characterization of pathogenic E. coli.
  38. Nataro, J. & Kaper, J. (1998). Diarrheagenic E. coli. Clinical Microbiology Reviews, 11,142-201. DOI: 10.1128/CMR.11.1.142
  39. Niranjan, K., Sathiyaseelan, V. & Jeyaseelan, E. C. (2013). Screening for anti-microbial and phytochemical properties of different solvents extracts of leafs of Porgamia Pinnata. International Journal of Scientific and research Publications, 3, 1-3.
  40. Rahman, M. A., Samad, M. A., Rahman, M. B. & Kabir, S. M. L. (2004). In vitro antibiotic sensitivity and therapeutic efficacy of experimental salmonellosis, colibacillosis and pasteurellosis in broiler chickens. Journal of Veterinary Medicine, 2, 99-102. https://www.veterinaryworld.org/Vol.9/July-2016/10.html
  41. Rajasekaran, S., Sivagnanam, K. & Subramanian, S. (2005). Modulatory effects of aloe vera leaf gel extract on oxidative stress in rats treated with streptozotocin. Journal of Pharmacology and Pharmacol, 57 (2), 241-246. DOI: https://doi.org/10.1211/0022357055416
  42. Raksha, B., Pooja, S. & Babu, S. (2014). Bioactive compounds and medical properties of aloe vera L.: An update. Journal of Plants Sciences, 2 (3): 102-107. DOI: 0.11648/j.jps.20140203.11
  43. Redda, Y. T., Kebede, E., Cruz, C., Gugsa, G., Awol, N. & Mengeste, B. (2014). Potential antibacterial activity of crude extracts from Aloe vera, Zingiber officinale and Vinca major Medicinal Plants. International Journal of Microbiological Research, 5 (3), 201-207. DOI:10.5829/idosi.ijmr.2014.5.3.86177
  44. Saguibo, J. & Elegado, F. (2012). Resistance profile of probiotic lactic acid bacteria against inhibitory effects of selected plant extracts. The Philippine agricultural Scientist. 95 (1), 0031-7454. https://www.ukdr.uplb.edu.ph/journal-articles/2286/
  45. Selbie, H. & Roy, R. (2015). Blue Ventures.
  46. Sepos, E. (2012). Standard Operating Procedure, Rotary Evaporator in the POWER Laboratory.
  47. Sen, B. H., Akdeniz, B. G. & Denizci, A. A. (2000). The effect of ethylenediamine-tetraecetic acid on C. albicans OOOE, 90, 651-655. DOI: 10.1067/moe.2000.109640
  48. Shahzad, K., Ahmad, R., Nawaz, S., Saeed, S., & Iqbal Z. (2009). Comparative antimicrobial activity of aloe vera gel on microorganisms of public health significance. https://onlinelibrary.wiley.com/doi/10.1002/vms3.488
  49. Sharma, S., Singh, L. & Singh, S. (2013). Comparative study between Penicillin and Ampicillin. Scolars Journal of Applied medical Sciences, 1 (4), 291-294. DOI: 10.36347/sjams.2013.v01i04.019 https://www.saspublishers.com/media/articles/SJAMS14291-294_CosOdSI.pdf
  50. Singh, S., Sharma, P. K., Kumar, N. & Dudhe, R. (2010). Biological activities of aloe vera. International Journal of Pharmacy and Technology.
  51. Sydiskis, R. J., Owen, D. G., Lohr, J., Rosler, K. H. & Blomster, R. N. (1991). Inactivation of enveloped viruses by anthraquinones extracted from plants. Antimicrobial Agents and Chemotherapy, 35 (12), 2463-2466. https://doi.org/10.1128/aac.35.12.2463
  52. Tanaka, M., Misawa, E., Ito, Y., Habara, N., Nomaguchi, K., Yamada, M., & Higuchi, R. (2006). Identification of five phytosterols from aloe vera gel as anti-diabetic compounds. Biological and Pharmaceutical Bulletin, 29, 1418-1422. DOI: 10.1248/bpb.29.1418
  53. Taiwo, V. O., Olunkule, O. A., Ozor, I. C. & Oyejobi, A. T. (2005). Consumption of aqueous extract of raw Aloe vera leaves: Histopathological and biochemical studies in rat and tilapia. African Journal of Biomedical Research, 8, 169-178. DOI: 10.4314/ajbr.v8i3.35746
  54. Thiruppathi, S., Ramasubramanian, V., Sivakumar, T. & Thinumalai Arasu, V. (2010). Antimicrobial activity of aloe vera (L.) Burm against pathogenic microorganisms. Journal of Biosciences Research, 1 (4), 251-258. https://www.researchgate.net/publication/285327171_Antimicrobial_activity_of_Aloe_vera_L_Burm_F_against_pathogenic_microganisms
  55. Tian, B., Hua, Y.J., Ma, X.Q. & Wang, G.L. (2003). Relationship between antibacterial activity of aloe and its anthraquinone compounds. China J. Chinese Material Medica 28, 1034-1037.
  56. Vidic, D., Taric, E., Alagic, J. & Maksimovic M. (2014). Determination of total phenolic content and antioxidant activity of ethanol extracts from Aloe spp. Bulletin of the Chemists and Technologists of Bosnia and Herzegovina, 42, 5-10.
  57. Vinson, J. A., Al Kharrat, H. & Andreoli, L. (2005). Effect of aloe vera preparations on human bioavailability of vitamins C and E. Phytomedicine, 12, 760-765.
  58. Wadhawan, R., Khan, S.D.A.A., Solanki, G. & Sabir, S. (2014). Aloe vera: A boon in Dentistry. International journal of Pharmacy Review and Research, 4 (3), 147-151.