Aira Janella L. Elec | John Albert P. Lachica | Eureka Teresa M. Ocampo
The Carabao mango is the Philippines’ vital and premium export variety because of its nutritive value and its distinctive sweet taste compare to other varieties. Carabao variety is one of the top fruit exports that has been essential in the growth of the Philippine economy. According to PSA (2015), mango ranks third in terms of production volume of 885,000 metric tons. Its export potential is, however, hampered by industry problems and a growing preference for mangoes with red blush. Red blush trait correlates to plant resistance against cold and pathogens and has a potential benefit to human health. Ten newly designed primers based on SNP of chalcone synthase gene derived from the GBS data of 341 mango genotypes were used to amplify chalcone synthase from sixteen mango genotypes with red and yellow skin color. This was an attempt to develop SNP markers for a red blush that can be utilized for mango breeding programs. Only four out of ten primers were successfully optimized for the amplification of chalcone synthase namely FLVS, Chs2, DRPRGA3, and At4g. The optimum annealing temperatures for each of the markers were 51.6°C, 49°C, 47.9°C, and 46°C, respectively, resulting in amplification of desired DNA bands after PCR. The primers were able to differentiate mangoes with red peels from those with yellow peels. After sequencing of selected PCR products followed by sequence analysis, it was found that red and yellow varieties exhibited highly conserved sequences for the chalcone synthase. However, different insertions and deletions within the conserved region were also observed. The phylogenetic tree based on Chs2 sequence of sixteen mango genotypes showed that the same-colored mangoes tended to cluster together in one clade, showing the usefulness of chalcone synthase SNPs for identifying red skin color in mango.
ISSN 2719-1990 (Print)
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