Philippine E-Journals

Oligosaccharide Profiling of Milk Colostrum from Two Breeds of Porcine

Jessica G. Asuncion | Connie A. Remoroza | Sara Yang | Tytus D. Mak | Yuxue Liang | Doreen D. Domingo | Prima Fe R. Franco | Shirley C. Agrupis | Stephen E. Stein

 

Abstract:

Colostrum oligosaccharides are important components of milk with multifunctional health benefits to newborn children. There is an increasing interest in porcine milk because of its similarity to the milk components in human milk. However, there is limited data on the oligosaccharide profile in porcine colostrum. The study focuses on the identification and annotation of colostrum oligosaccharides in two breeds of porcine using liquid chromatography-mass spectrometry and NIST mass spectral library search methods. One-hundred sixty-six oligosaccharides were identified and used to build a glycan mass spectral library of porcine milk. Comparing PMO (Porcine milk oligosaccharides) profile using NIST glycan library repository, 63 is found unique to porcine, 79 are oligosaccharides that shared a common structure with mature human milk and 24 are common to other mature mammalian milk. The PMO displayed different patterns of variation between black and white breeds. Of which, PMO content is highest in the black breed, giving 12 unique oligosaccharides that are not found in the white breed. In general, porcine milk contains both acidic (sialylated) and neutral (fucosylated) oligosaccharides, but oligosaccharides containing sialic acid predominate the PMO profile in both breeds. Among the identified oligosaccharide, pLNnH (neutral) and 3’-Sialyllactose (acidic) is the most abundant in both breeds. In summary, colostrum from the black breed has the best oligosaccharide profile and diversity.