Identification of Non-Peptidic Venom Components of Philippine Tarantula Species

Olga  M. Nuñeza,; Leonardo  A. Guevarra,; Ralph Emerson John  Molino,; Anna Beatriz  R. Mayor,; Mark Kevin  A. Devanadera,; Camille  Rodrigues,; Myla  R. Santiago-bautisa,; Gardee  T. Pena,; Hiyas  A. Junio,

Identification of Non-Peptidic Venom Components of Philippine Tarantula Species

Abstract:

There is a growing interest in the non-peptidic components of spider venom because of the neurotoxic and cytotoxic activities of this group of compounds. In this study, we aimed to identify the non-peptidic venom components of tarantula species collected from the Municipality of Wao in Lanao del Sur in Mindanao. Spider venom was extracted by electrostimulation for untargeted metabolomics analysis. Initial separation of the components of the venom was done by reverse-phase high-performance liquid chromatography (RP-HPLC). A fraction previously reported to contain non-peptidic bioactive components were further analyzed by Ultra Performance Liquid Chromatography – Quadrupole Time of Flight Data Dependent Analysis (UPLC-QTOF DDA) to structurally identify the components.