Alpha-Amylase PRoduction in Shake Flask Culture of Bacillus subtilis NRRLB3411

Ernesto  J. del Rosario,; Claudia  Valdeavella,

Alpha-Amylase PRoduction in Shake Flask Culture of Bacillus subtilis NRRLB3411

Claudia Valdeavella | Ernesto J. del Rosario

Discipline: Biological and Sport Sciences

Abstract:

Bacillus subtilis NRRL B3411 was found to give the highest yield of alpha-amylase in shake-flask culture in comparison with Bacillus licheniformis NRRL 1001 and a local isolate of Bacillus amyloliquefaciens. The cultural and nutritional requirements for alpha-amylase production by this organism were determined. Peptone was the ideal nitrogen source. Optimal values of the pH, temperature, and period of incubation for alpha-amylase formation were 6.5-7.5,40°C, and five days, respectively.

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