Superoxide Generation In Catfish Liver Microsomes Treated With Menadione

Armando  A. Andaya,

Superoxide Generation In Catfish Liver Microsomes Treated With Menadione

Abstract:

The study investigated the possible involvement of oxygen free radicals in mediating the toxicity of menadione (2-methyl-1,4-naphthoqoinone) in channel catfish lctalurus pU11cta1us L. under laboratory exposure conditions. It was hypothesized that menadione is metabolized via a flavoenzyme NADPH-cytochrome P-450 reductase resulting in the production of semiquinones which under aerobic condition generar.es toxic oxygen species including superoxide anions, hydrogen peroxide, and singlet oxygen. The probable existence of this flavoenzyme in catfish liver~ was iooica(ed and its possible involvement in lhe metabolism of menadione was demonstral.Cd. The concentrationdependent rates of menadione-induced cyt.ochromc c reduction and oxygen consumption provided evidences. for lhe presence of superoxide generating system in catfish liver miaosooies. These obser• vat.ions validare the claim that oxygen free radicals could be involved, at least in part, in the mode of toxic action of menadione in channel catfish.

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