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HomePhilippine Journal of Veterinary and Animal Sciencesvol. 39 no. 1 (2013)

Optimized Extenders for Cryopreservation of Buck Semen for artificial Insemination

Ma. Asuncion G. Beltran | Eufrocina P. Atabay, Dvsm, Mvs, Phd | Edwin C. Atabay | Emilio M. Cruz | Flocerfida P. Aquino | Libertado C. Cruz

Discipline: Veterinary Medicine, Animal Science



The study was conducted to evaluate the efficiency of different nonpermeating cryoprotectants for freezing buck semen. Semen samples were collected from three healthy bucks and were evaluated for volume, color, consistency, pH, motility and concentration. Samples with 60% and above motility were allocated to the following treatments of Tris-Citric Acid-Fructose –Raffinose-Glycerol-based extenders: T1 – 5% egg yolk; T2 – 10% egg yolk; T3 – 15% egg yolk; T4 – 20% egg yolk; T5 – 2.5% goat serum; T6 – 5% goat serum; T7 – 10% goat serum; T8 – 10mg Bovine Serum Albumin (BSA); T9 – 30 mg BSA; and T10 – 50 mg BSA. Post-thawing evaluation revealed that extenders with 5% egg yolk (T1), 2.5% goat serum (T5), and 30 mg BSA (T9) yielded high rates of sperm motility and acrosome integrity. In terms of the proportion of live sperm at post thawing, 5% egg yolk was found significantly higher than 2.5% goat serum and 30 mg BSA. Moreover, cost analysis revealed that supplementation with 5% egg yolk was cheaper than with goat serum and BSA. Artificial insemination (AI) using frozen-thawed semen with 5% egg yolk resulted in 70.50% birthrate. This work demonstrates the successful production of kids from frozen buck semen enhancing the potential of AI technology for goat production.