HomeIAMURE International Journal of Science and Clinical Laboratoryvol. 7 no. 1 (2015)

Blood Cell Increasing Activity and Nutritional Value of Formulated Suspension from Guyabano (Annona muricata L., Annonaceae) Crude Leaf Extract

Marilou L. Vicente-tablizo

Discipline: Science



Lowering of blood elements erythrocytes, leukocytes, and thrombocytes is a major concern in the treatment of cancers. There is a need to discover natural agents that improve blood condition. This study investigated the blood cell increasing activity and nutritional value of a formulated suspension from Guyabano (Annona muricata L., Annonaceae) crude leaf extract. It also determined the extract’s Approximate Effective Dose (AED) and Median Effective Dose (ED50) as erythrocyte, leukocyte and thrombocyte increasing agent by log dose interval. Results show that Guyabano (A. muricata) crude leaf extract was non-toxic even at dose level of 2000 mg/Kg body weight; hence, it can be considered safe, under Category 5 of OECD Guidelines. The AED of the Guyabano (A. muricata) crude leaf extract was 1–3.98 mg/kg body weight and 3.98–15.84 mg/kg body weight on increasing erythrocytes and leukocytes, respectively; while the extract’s AED on increasing the thrombocytes was indeterminate. As for the Effective Dose on the 50% (ED50) of test rabbits, the Guyabano (A. muricata) extract showed erythrocyte increasing activity at 16.93 mg/kg body weight; leukocyte increasing activity ED50 at 10.60 mg/kg body weight; while thrombocyte increasing activity ED50 at 18.03 mg/kg body weight. The formulated suspension of Guyabano (A. muricata) crude leaf extract contains protein, sodium, iron, potassium, calcium, magnesium, zinc and phosphorus.