Home⇛NRCP Research Journal⇛vol. 24 no. 2 (2025)

Acinetobacter spp. Dominate the Competing Microbiota of Campylobacter spp. in Selective Medium

Eleanor S. Austria | Christian Paul Munoz | Jhasmine Lyndsay Falogme | Marinette Rose M. Carpio | Jarale Joy Cabagbag | Kian Christian J. Elman | Gabriel Alexis S. Tubalinal | Claro N. Mingala

Discipline: food sciences

 

Abstract:

Non-target microorganisms grow abundantly on selective media presenting difficulty in the isolation of the more fastidious Campylobacter. This study aims to identify the competing microbiota of Campylobacter on modified charcoal cefoperazone agar, to help in the future development of protocol for isolating Campylobacter spp. Raw milk from backyard-raised carabaos were analyzed for the presence of Campylobacter spp. and the non-Campylobacter spp. that grew on the selective medium were identified using 16S rRNA and gene sequencing. Ten (10) bacterial strains were detected including, Acinetobacter spp., Escherichia coli, Pseudomonas spp. and Klebsiella pneumonia. Thirty (30) of the 53 sequenced isolates were identified as Acinetobacter spp. and eleven (11) were E. coli. Further analysis of confirmed Acinetobacter spp. isolates with PCR and blaOXA51-like gene primer revealed that majority (18 of 20 isolates) were A. baumanii. This study showed the composition of the competing microflora of Campylobacter on mCCDA, a data that can be used to determine the appropriate conditions and supplements to be added to the media to successfully isolate Campylobacter jejuni and C. coli in the raw milk of carabaos. Furthermore, this study revealed the presence of possible pathogenic microorganisms in raw carabaos’ milk which underscored the need to observe proper milk handling and processing to ensure public health safety.



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